The Potential of CRISPR
Abstract: The newly developed technique, CRISPR, or Clustered Regularly Interspaced Short Palindromic Repeats, is based on the bacterial “immune system”. CRISPR-Cas9 system was discovered in the 1970s in the E.Coli bacteria. This system is naturally found in the bacteria as an anti-infection mechanism. If an analogy is to be drawn, the CRISPR-Cas9 system can be compared to the antibodies in humans. When the bacteria sense a viral invasion, it targets the viral DNA and creates strands of this targeted DNA. These fragments are known as the “CRISPR fragments” and are stored as a memory when there is an invasion by the same virus. If there is an invasion by the same virus, the CRISPR fragments produce RNA which later targets the viral DNA. The Cas9 enzyme functions to destroy the viral DNA. Similarly, in the lab, a biologist can target a specific sequence of the gene and use the CRISPR “scissors” to cut the DNA at a specific site. Scientists aim to apply this technique for cancer ...